{"id":333,"date":"2007-05-20T00:00:00","date_gmt":"1970-01-01T00:00:00","guid":{"rendered":"http:\/\/wptest.psych.ualberta.ca\/~cdickson\/wordpress\/?p=333"},"modified":"2007-05-20T00:00:00","modified_gmt":"1970-01-01T00:00:00","slug":"immunohistochemical-characterization-of-substance-p-receptor-nk1r-expressing-interneurons-in-the-entorhinal-cortex","status":"publish","type":"post","link":"https:\/\/sites.psych.ualberta.ca\/brainrhythmslab\/immunohistochemical-characterization-of-substance-p-receptor-nk1r-expressing-interneurons-in-the-entorhinal-cortex\/","title":{"rendered":"Immunohistochemical characterization of substance P receptor (NK(1)R)-expressing interneurons in the entorhinal cortex"},"content":{"rendered":"<p>PMID: <a href='http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/17366610' title=''>17366610<\/a><\/a><\/p>\n<p>Wolansky T, Pagliardini S, Greer JJ, Dickson CT<\/p>\n<p>J. Comp. Neurol. 2007 May;502(3):427-41<\/p>\n<h2>Abstract<\/h2>\n<p><p>It has been reported that application of substance P (SP) to the medial portion of the entorhinal cortex (EC) induces a powerful antiepileptic effect (Maubach et al. [1998] Neuroscience 83:1047-1062). This effect is presumably mediated via inhibitory interneurons expressing the neurokinin-1 receptor (NK(1)R), but the existence of NK(1)R-expressing inhibitory interneurons in the EC has not yet been reported. The present immunohistochemical study was performed in the rat to examine the existence and distribution of NK(1)R-expressing neurons in the EC as well as any co-expression of other neurotransmitters\/neuromodulators known to be associated with inhibitory interneurons: gamma-aminobutyric acid (GABA), parvalbumin (PARV), calretinin (CT), calbindin (CB), somatostatin (SST), and neuropeptide Y (NPY). Our results indicated that NK(1)R-positive neurons were distributed rather sparsely (especially in the medial EC), primarily in layers II, V, and VI. The results of our double-immunohistochemical staining indicated that the vast majority of NK(1)R-expressing neurons also expressed GABA, SST, and NPY. In addition, CT was co-expressed in a weakly stained subgroup of NK(1)R-expressing neurons, and CB was co-expressed very rarely in the lateral EC, but not in the medial EC. In contrast, SP-immunopositive axons with fine varicosities were distributed diffusely throughout all layers of the EC, appearing to radiate from the angular bundle. SP may be released in a paracrine manner to activate a group of NK(1)R-expressing entorhinal neurons that co-express GABA, SST, and NPY, exerting a profound inhibitory influence on synchronized network activity in the EC.<\/p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>PMID: 17366610 Wolansky T, Pagliardini S, Greer JJ, Dickson CT J. Comp. Neurol. 2007 May;502(3):427-41 Abstract It has been reported that application of substance P (SP) to the medial portion of the entorhinal cortex (EC) induces a powerful antiepileptic effect (Maubach et al. [1998] Neuroscience 83:1047-1062). This effect is presumably mediated via inhibitory interneurons expressing [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-333","post","type-post","status-publish","format-standard","hentry","category-uncategorized","post-preview"],"_links":{"self":[{"href":"https:\/\/sites.psych.ualberta.ca\/brainrhythmslab\/wp-json\/wp\/v2\/posts\/333","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.psych.ualberta.ca\/brainrhythmslab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.psych.ualberta.ca\/brainrhythmslab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.psych.ualberta.ca\/brainrhythmslab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.psych.ualberta.ca\/brainrhythmslab\/wp-json\/wp\/v2\/comments?post=333"}],"version-history":[{"count":0,"href":"https:\/\/sites.psych.ualberta.ca\/brainrhythmslab\/wp-json\/wp\/v2\/posts\/333\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.psych.ualberta.ca\/brainrhythmslab\/wp-json\/wp\/v2\/media?parent=333"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.psych.ualberta.ca\/brainrhythmslab\/wp-json\/wp\/v2\/categories?post=333"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.psych.ualberta.ca\/brainrhythmslab\/wp-json\/wp\/v2\/tags?post=333"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}